Biotinylated Human IL-17A&F Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F) [Accession | Q16552-1(IL-17A)&Q96PD4(IL-17F)].

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.1 kDa (IL-17A) and 17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa and 27 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human IL-17A&F on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

ELISA Data

Immobilized Human IL-17R alpha, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.30μg/ml determined by ELISA.

背景(Background)

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

分子别名(Synonyms)

IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F

文献(References)

(1)Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.