ILR-HM111 | 价格

ILR-HM111-100μg / 询价

ILR-HM111-500μg / 询价

ILR-HM111-500μgx2 / 询价

Human IL-11R alpha Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-11R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ser24-Ala370 [Accession | Q14626-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 38.8 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-11R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-11R alpha is greater than 95% as determined by SEC-HPLC.

SPR Data

Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).

背景(Background)

Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.

分子别名(Synonyms)

IL-11R subunit alpha; IL-11R-alpha; IL-11RA; IL11RA

文献(References)

(1) Onnis B, Fer N, Rapisarda A, Perez VS, Melillo G. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells. J Clin Invest. 2013 Apr;123(4):1615-29. doi: 10.1172/JCI59623. Epub 2013 Mar 15. PMID: 23549086; PMCID: PMC3613900.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.