ILR-HM4R1B | 价格

ILR-HM4R1B-100μg / 询价

ILR-HM4R1B-500μg / 询价

ILR-HM4R1B-500μgx2 / 询价

Biotinylated Human IL-13Ra1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human IL-13Ra1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Ala27-Thr343 [Accession | P78552-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human IL-13Ra1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated IL-13Ra1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human IL-13Ra1, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA (QC Test).

背景(Background)

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

分子别名(Synonyms)

IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074

文献(References)

(1) Suzuki A , Leland P , Joshi B H , et al. Targeting of IL-4 and IL-13 receptors for cancer therapy[J]. Cytokine, 2015, 75(1):S1043466615002124.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.