货号价格

LIL-HM4B1B-100μg / 询价

LIL-HM4B1B-500μg / 询价

LIL-HM4B1B-500μgx2 / 询价

Biotinylated Human LILRB1/CD85j/ILT2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly24-His458.[Accession | Q8NHL6-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human LILRB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human LILRB1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human LILRB1, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.

背景(Background)

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.

分子别名(Synonyms)

ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4

文献(References)

(1)Anderson K J , Allen R L. Regulation of T‐cell immunity by leucocyte immunoglobulin‐like receptors: innate immune receptors for self on antigen‐presenting cells[J]. Immunology, 2009, 127.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.