MHC-HM443-100μg / 询价
MHC-HM443-500μg / 询价
MHC-HM443-500μgx2 / 询价
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Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein
Recombinant Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide [Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLQHLIGL].
The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 51-58 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Monomer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR with the EC50 of 55.4ng/ml determined by ELISA.
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
PRAME; OIP-4; OIP4; MAPE;
Lezcano C, Jungbluth AA, Busam KJ. PRAME Immunohistochemistry as an Ancillary Test for the Assessment of Melanocytic Lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001. Epub 2021 Apr 28. PMID: 34023098; PMCID: PMC8152939.
