恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Human HLA-A02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide [Accession \| P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL].
分子量大小（Molecular Weight）	The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
纯度（Purity）	> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储（Storage）	-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

表达区间及表达系统（Source）

Recombinant Human HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide [Accession | P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL].

分子量大小（Molecular Weight）

The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.

纯度（Purity）

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储（Storage）

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.