NEC-HM403-100μg / 询价
NEC-HM403-500μg / 询价
NEC-HM403-500μgx2 / 询价
Human Nectin-3/CD113 Protein
Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400 [Accession | Q9NQS3].
The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Nectin-3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200).
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
(1) Inagaki, M. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development[J]. Development, 132(7):1525-1537.
