NRG-HM201 | 价格

NRG-HM201-100μg / 询价

NRG-HM201-500μg / 询价

NRG-HM201-500μgx2 / 询价

Human NRG1 Beta 1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human NRG1 Beta 1 Protein is expressed from HEK293 with hFc tag at the N-terminus.
It contains Ser2-Lys246 [Accession | Q02297-6].

分子量大小(Molecular Weight)

The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Her4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human Her3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.

背景(Background)

Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.

分子别名(Synonyms)

Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A

文献(References)

Liew H, Kim YM, Choi HS, Jang AR, Churchill D, Lee SH, Suh YH. Soluble Neuregulin-1 from Microglia Enhances Amyloid Beta-induced Neuronal Death. CNS Neurol Disord Drug Targets. 2016;15(8):918-926. doi: 10.2174/1871527315666160815160505. PMID: 27577739.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.