HER-HM4B4 | 价格

HER-HM4B4-100μg / 询价

HER-HM4B4-500μg / 询价

HER-HM4B4-500μgx2 / 询价

Human Her4/ErbB4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Her4/ErbB4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gln26-Pro651 [Accession | Q15303-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Her4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Her4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Her4 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 34.7ng/ml determined by ELISA.

背景(Background)

Her4, a member of the EGF receptor family, plays a variety of roles in physiological and pathological states. Genetic studies have indicated a link between Her4 and type 2 diabetes and obesity. Her4 may play an important role in glucose homeostasis and lipogenesis. Her4 deficiency-related obesity and adipose tissue inflammation may contribute to the development of MetS.

分子别名(Synonyms)

p180erbB4; 4ICD; E4ICD; HER4; ErbB4; MGC138404; ALS19

文献(References)

(1)Zeng F, Wang Y, Kloepfer LA, Wang S, Harris RC. Her4 deletion predisposes to development of metabolic syndrome in mice. Am J Physiol Endocrinol Metab. 2018 Oct 1;315(4):E583-E593. doi: 10.1152/ajpendo.00166.2018. Epub 2018 Jun 26. PMID: 29944391; PMCID: PMC6230712.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.