PD1-HM101-100μg / 询价
PD1-HM101-500μg / 询价
PD1-HM101-500μgx2 / 询价
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Biotinylated Human PD-L1/B7-H1 Protein
Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Cynomolgus PD-L1/B7-H1 Protein
Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling)
Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling)
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Human PD-1/PDCD1 Protein
Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1]
The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human PD-1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 2.20 μM as determined in SPR assay (Biacore T200).
Human PD-L2, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
PDCD1; PD1; CD279; SLEB2; PD-1
(1)Blank C , Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.
