PD1-HM201-100μg / 询价
PD1-HM201-500μg / 询价
PD1-HM201-500μgx2 / 询价
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关联产品
Biotinylated Human PD-L1/B7-H1 Protein
Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein
Cynomolgus PD-L1/B7-H1 Protein
Biotinylated Cynomolgus/Rhesus macaque PD-L1/B7-H1 Protein (Primary Amine Labeling)
Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling)
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Human PD-1/PDCD1 Protein
Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167 [Accession | Q15116-1].
The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human PD-L1, mFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA (QC Test).
Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 μM as determined in SPR assay (Biacore T200).
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
PDCD1; PD1; CD279; SLEB2; PD-1
(1)Blank C , Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.
