PTP-HM104-100μg / 询价
PTP-HM104-500μg / 询价
PTP-HM104-500μgx2 / 询价
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Human CD45/PTPRC Protein
Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Lys577 [Accession | P08575-3].
The protein has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 118-150 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD45 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD45, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 78.1ng/ml determined by ELISA (QC Test).
PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
(1) Courtney AH, Shvets AA, Lu W, Griffante G, Mollenauer M, Horkova V, Lo WL, Yu S, Stepanek O, Chakraborty AK, Weiss A. CD45 functions as a signaling gatekeeper in T cells. Sci Signal. 2019 Oct 22;12(604):eaaw8151. doi: 10.1126/scisignal.aaw8151. PMID: 31641081; PMCID: PMC6948007.
