SMD-HB101-100μg / 询价
SMD-HB101-500μg / 询价
SMD-HB101-500μgx2 / 询价
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Human SMPD1 Protein
Recombinant Human SMPD1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Pro628 [Accession | NP_000534.3].
The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human SMPD1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SMPD1 is greater than 95% as determined by SEC-HPLC.
Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/μg, as measured under the described conditions.
Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.
ASM;
Ueda S, Manabe Y, Kubo N, Morino N, Yuasa H, Shiotsu M, Tsuji T, Sugawara T, Kambe T. Early secretory pathway-resident Zn transporter proteins contribute to cellular sphingolipid metabolism through activation of sphingomyelin phosphodiesterase 1. Am J Physiol Cell Physiol. 2022 May 1;322(5):C948-C959. doi: 10.1152/ajpcell.00020.2022. Epub 2022 Mar 16. PMID: 35294847.
