Cynomolgus TREM2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus TREM2 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains His19-Ser174 [Accession | A0A2K5UY39-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 33-42 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus TREM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Cynomolgus TREM2, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 50.3ng/ml determined by ELISA.

背景(Background)

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2  consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.

分子别名(Synonyms)

PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c

文献(References)

(1)Ford J W , Mcvicar D W. TREM and TREM-like receptors in inflammation and disease[J]. Current Opinion in Immunology, 2009, 21(1):38-46.