Mouse APOE/Apolipoprotein E Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse APOE/Apolipoprotein E Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Glu19-Gln311 [Accession | P08226].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.1 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse APOE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SPR Data

Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).

背景(Background)

Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

分子别名(Synonyms)

Apolipoprotein E; Apo-E; APOE; apolipo E

文献(References)

(1) Zhao N, Liu CC, Qiao W, Bu G. Apolipoprotein E, Receptors, and Modulation of Alzheimer's Disease. Biol Psychiatry. 2018 Feb 15;83(4):347-357. doi: 10.1016/j.biopsych.2017.03.003. Epub 2017 Mar 14. PMID: 28434655; PMCID: PMC5599322.