TRP-CM121 | 价格

TRP-CM121-100μg / 询价

TRP-CM121-500μg / 询价

TRP-CM121-500μgx2 / 询价

Cynomolgus TROP-2/TACSTD2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains His27-Thr274 [Accession | XP_005543292.1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus TROP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus TROP-2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus TROP-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.

背景(Background)

Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.

分子别名(Synonyms)

EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2

文献(References)

(1)Zimmers SM, Browne EP, Williams KE, et al. TROP2 methylation and expression in tamoxifen-resistant breast cancer. Cancer Cell Int. 2018;18:94. Published 2018 Jul 6. doi:10.1186/s12935-018-0589-9

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.