2B4-HM401B-100μg / 询价
2B4-HM401B-500μg / 询价
2B4-HM401B-500μgx2 / 询价
Biotinylated Human 2B4/CD244/SLAMF4 Protein
Recombinant Biotinylated Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Cys22-Arg221 [Accession | Q9BZW8-2].
The protein has a predicted MW of 24.9 kDa. Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human 2B4 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA.
2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
(1)Velikovsky C A , Deng L , Chlewicki L K , et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584.
