CD8-HM428B | 价格

CD8-HM428B-100μg / 询价

CD8-HM428B-500μg / 询价

CD8-HM428B-500μgx2 / 询价

Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.
It contains Asn19-Pro152 [Accession | P10747].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Anti-CD28 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test).

SPR Data

Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 μM as determined in SPR assay (Biacore T200).

背景(Background)

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

分子别名(Synonyms)

CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290

文献(References)

(1)Suntharalingam G , Perry M R , Ward S , et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028.