B71-MM180 | 价格

B71-MM180-100μg / 询价

B71-MM180-500μg / 询价

B71-MM180-500μgx2 / 询价

Mouse B7-1/CD80 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse B7-1/CD80 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Val38-Asn246 [Accession | Q00609-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse B7-1 is greater than 95% as determined by SEC-HPLC.

背景(Background)

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

分子别名(Synonyms)

CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

文献(References)

(1) Merwe V D , P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.D

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.