Human Nectin-2/CD112 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Nectin-2/CD112 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln32-Leu360 [Accession | Q92692-2].

分子量大小(Molecular Weight)

The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.

SPR Data

Human Nectin-2, hFc Tag captured on CM5 Chip via Protein A can bind Human PVRIG, His Tag with an affinity constant of 6.27 μM as determined in SPR assay (Biacore T200).

背景(Background)

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

分子别名(Synonyms)

CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2

文献(References)

(1) Bekes I, Löb S, Holzheu I, Janni W, Baumann L, Wöckel A, Wulff C. Nectin-2 in ovarian cancer: How is it expressed and what might be its functional role? Cancer Sci. 2019 Jun;110(6):1872-1882. doi: 10.1111/cas.13992. Epub 2019 May 2. PMID: 30843637; PMCID: PMC6549928.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.