PGL-HM201 | 价格

PGL-HM201-100μg / 询价

PGL-HM201-500μg / 询价

PGL-HM201-500μgx2 / 询价

Human PGLYRP1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human PGLYRP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln22-Pro196 [Accession | O75594].

分子量大小(Molecular Weight)

The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human PGLYRP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PGLYRP1 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human PGLYRP1, hFc Tag captured on CM5 Chip via Protein A can bind Human TREM1, His Tag with an affinity constant of 17.39 µM as determined in SPR assay (Biacore T200).

背景(Background)

Innate immunity protein Tag7 (PGRP-S, PGLYRP1) can interact with the TNFα receptor, TNFR1, and block the transduction of apoptotic signals through this receptor. A complex formed between the Tag7 protein and the major heat shock protein Hsp70 can activate TNFR1 receptor and induce tumor cell death via either apoptotic or necroptotic pathway.

分子别名(Synonyms)

PGRP-S; PGLYRP1; PGLYRP; PGRP; TNFSF3L; PGRP-SMGC126894; PGRPSMGC126896; TAG7; Tasg7

文献(References)

(1) Romanova EA, Sharapova TN, Telegin GB, Minakov AN, Chernov AS, Ivanova OK, Bychkov ML, Sashchenko LP, Yashin DV. A 12-mer Peptide of Tag7 (PGLYRP1) Forms a Cytotoxic Complex with Hsp70 and Inhibits TNF-Alpha Induced Cell Death. Cells. 2020 Feb 20;9(2):488. doi: 10.3390/cells9020488. PMID: 32093269; PMCID: PMC7072780.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.