CTL-HM241-100μg / 询价
CTL-HM241-500μg / 询价
CTL-HM241-500μgx2 / 询价
Human CTLA-4/CD152 Protein
Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys36-Asp161 [Accession | P16410-1].
The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Immobilized Human B7-1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
(1)Merwe V D , P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.
