Cynomolgus CTLA-4/CD152 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ala37-Ser160 [Accession | G7PL88-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.

SPR Data

Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).

背景(Background)

CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

分子别名(Synonyms)

CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12

文献(References)

(1)Merwe V D , P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.