B72-CM186 | 价格

B72-CM186-100μg / 询价

B72-CM186-500μg / 询价

B72-CM186-500μgx2 / 询价

Cynomolgus B7-2/CD86 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-terminus.
It contains Tyr25-His239 [Accession | A0A2K5TTJ8].

分子量大小(Molecular Weight)

The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus B7-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus B7-2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus B7-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).

SPR Data

Cynomolgus B7-2, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 0.102 μM as determined in SPR assay (Biacore T200).

背景(Background)

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

分子别名(Synonyms)

CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2

文献(References)

(1) Bhatia S, Edidin M, Almo SC, Nathenson SG. B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Immunol Lett. 2006 Apr 15;104(1-2):70-5. doi: 10.1016/j.imlet.2005.11.019. Epub 2005 Dec 13. PMID: 16413062.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.