HER-MM103 | 价格

HER-MM103-100μg / 询价

HER-MM103-500μg / 询价

HER-MM103-500μgx2 / 询价

Mouse Her3/ErbB3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse Her3/ErbB3 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ser20-His641 [Accession | Q61526].

分子量大小(Molecular Weight)

The protein has a predicted MW of 69.59 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse Her3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse Her3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse Her3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).

背景(Background)

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

分子别名(Synonyms)

ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3

文献(References)

(1)Yonesaka K , Haratani K , Hirotani K , et al. Abstract 44: U3-1402, a novel HER3-targeting ADC, and a novel DNA topoisomerase I inhibitor inhibit the growth of non-small cell lung cancer with EGFR mutation[J]. Cancer Research, 2017, 77(13 Supplement):44-44.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.